Polyclonal & Monoclonal custom antibody production services
Complete peptide polyclonal antibody package (17-20 weeks)
- Peptide synthesis (<15 a.a., >85% purity, 10 mg)
- A cysteine is automatically added to the C terminus (or N terminus) of the peptide and conjugated to KLH.
- Two rabbits, pre-immune bleed, four immunizations/rabbit
- Final bleed
- ELISA test with report
- Lyophilization
- 2 ml of pre-immune serum supplied
- 100 ml of antisera (lyophilized) from two rabbits
- Approx 5mg of unconjugated peptide, MS and HPLC reports
Complete affinity-purified polyclonal antibody package (17-20 weeks)
- Peptide synthesis (<15 a.a., >85% purity, 15 mg)
- A cysteine is automatically added to the C terminus (N terminus) of the peptide and conjugated to KLH.
- Two rabbits, pre-immune bleed, four immunizations/rabbit
- Final bleed
- Peptide (5 mg) conjugated to affinity resin
- Affinity purified antibodies
- ELISA test with report
- Lyophilization
- 1-2 ml of pre-immune serum
- 3-50 mg affinity-purified antibodies (lyophilized)
- Approx 5 mg of unconjugated peptide, MS and HPLC reports
Phospho-Specific Polyclonal Antibody Package (17-20 weeks)
- Synthesis of two peptides, one with modification (<15 a.a, >90% purity, 15 mg)and the other without modification (<15 a.a, >90% purity, 10 mg)
- A cysteine is automatically added to the C terminus (N terminus) of the peptide and conjugated to KLH.
- Two rabbits, pre-immune bleed, four immunizations/rabbit
- Prepare two affinity columns
- Antibody purification by modified-peptide affinity column and unmodified-peptide cross-adsorption.
- ELISA test with report
- Lyophilization
- 1-30 mg phospho-specific antibodies (lyophilized) from two rabbits
- 5 mg of unconjugated phospho peptides and 5 mg of unconjugated non-phospho peptides
- 1-2 ml of pre-immune serum
Express service available wherein antibody shall be ready in 2 months
Custom Monoclonal Antibody Services
Basic Monoclonal Antibody Package (Mouse/Rat) 4-6 months
- Immunization and ELISA screening
- Fusion and screening
- Subcloning, expansion, cryopreservation and delivery
- Up to 5 positive hybridoma clones
- Two vials of cell culture for each clone
- 5 ml of culture supernatant
Complete Peptide Monoclonal Antibody Package (Mouse/Rat) 5-7 months
- Peptide synthesis
- Immunization and ELISA screening
- Fusion and screening
- Subcloning, expansion, cryopreservation and delivery
- Up to 5 positive hybridoma clones
- Two vials of cell culture for each clone
- 5 ml of culture supernatant
- 5 mg of unconjugated peptide
Phospho-Specific Monoclonal Antibody Package (Mouse/Rat) 5-7 months
- Peptide synthesis
- Immunization and ELISA screening
- Fusion and screening
- Subcloning, expansion, cryopreservation and delivery
- Up to 5 positive hybridoma clones
- Two vials of cell culture for each clone
- 5 ml of culture supernatant
- 5 mg of phospho-peptide
- 5 mg of unmodified peptide
Express service available wherein antibody shall be ready in 2.5 months