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Library Construction

Biotech Desk Pvt. Ltd. has tied up with Amplicon Express to provide the Indian scientist unrivaled expertise for BAC library construction. Your future goal may be to prepare a library for a full genome sequencing project or to seek a few BAC clones of interest, or numerous targets. You may or may not require these clones on nylon filters.

BAC Library:

  • HMW DNA shall be extracted.
  • Size of each insert varies from 100 to 180 kb depending upon the organism.
  • Coverage is guaranteed.
  • Experience with plant, fungal, bacterial and animal libraries.
  • Experience with GC-rich organisms and marine bacteria.
  • Client will receive original library and may request for additional copies.
  • Clones will be arrayed and amplified in 384-well plates (1 unique clone/well), and shipped on dry ice.
  • Amount of clones received is based on a guaranteed coverage and 120Kb average insert size.
  • Nylon filters available as an option for further screening.
  • Screening of BAC libraries can be also performed.

FOSMID Library:

  • Hydroshear technology to extract ~40kb fragments without cloning bias.
  • Suitable for samples for which HMW DNA cannot be contained such as metagenomic samples and some special environmental samples.
  • Coverage is guaranteed.
  • Client will receive original library and may request for additional copies.
  • Clones will be arrayed and amplified in 384-well plates (1 unique clone/well), and shipped on dry ice.
  • Amount of clones received is based on a guaranteed coverage and 40kb average insert size.
  • Nylon filters available as an option for further screening.
  • Screening of libraries can be also performed.

Metagenomic Library:

  • ~40kb fragments are isolated without cloning bias.
  • Coverage is guaranteed.
  • Client will receive original library and may request for additional copies.
  • Clones will be arrayed and amplified in 384-well plates (1 unique clone/well), and shipped on dry ice.
  • Amount of clones received is based on a guaranteed coverage and 40kb average insert size.
  • Nylon filters available as an option for further screening.
  • Screening of libraries can be also performed.

Library preparation for NGS:

  • Pacbio.
  • BioNano
  • Illumina
  • Gemcode

Special shipping instructions given for sending samples

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