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Recombinant Antibodies

Initially when Monoclonal antibodies were discovered there was a great hope for clinical use of antibodies. But this excitement gave way to pessimism as the antibodies derived from mouse induced unwanted immune response when subjected to humans. For this various technologies like display, humanization and transgenic animals have allowed Antibody based drugs that can overcome the Human Immune system. Recombinant antibodies are ideal for therapeutics because of their long half life, amenability to manipulation and improved binding to antigen.

Genscript can produce recombinant antibodies or rAbs using licensed technologies from NRC for transient and stable mammalian expression in HEK293 & CHO cell lines. The customer provides the antibody sequence for both Light and Heavy Chains. >95% purity is ensured by SDS-PAGE/HPLC with Endotoxin levels <1-10 EU/mg depending upon the quantity of rAb required.

Advantages:

  Recombinant antibody Traditional antibody

Production method

Recombinant technology

Cell/tissue/animal immunization

Production time

5-10 weeks for MamPower™ guaranteed
recombinant antibody production

Several months

Application

ELISA, WB, IP, IHC, IF, diagnostic, vaccine

ELISA,WB,IP,IHC,IF

Reproducibility

Yes

No

Customizable

Yes

No

Isotype Switching Possible

Yes

No

Against toxic
/non-immunogenic proteins

Yes

No

Removal or minimization of animal use

Yes

No

Tagged with specific markers

Yes

No

Format

IgG, Fab, VHH, ScFv

IgG

MamPowerâ„¢ guaranteed recombinant antibody production services

Customer provides

Required recombinant antibody amount

Purity & endotoxin level options

Timeline

Deliverables

Price

Antibody sequence

5mg

· ≥95% by SDS-PAGE

5-6 weeks

· LC and HC in pUC57 vector

· Optimized gene sequence report

· Purified antibody at listed amount and purity

· QC data

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10mg
25mg
50mg
100mg

· ≥95% by SDS-PAGE

· Endotoxin ≤10EU/mg

· Concentration ≥1mg/ml

6-8 weeks

250mg
500mg

· ≥95% by HPLC

· Endotoxin ≤1EU/mg

8-10 weeks

Work flow of this service include-

  • Sequence evaluation, codon optimization & HTP gene synthesis
  • Cloning in expression vector, plasmid prep
  • High throughput transient transfection
  • Expression screening, Affinity purification
  • Guarantee rAb quantity, purity & endotoxin control
  • Flexible scale from 5 mg- 500 mg

Genscript offers choice of E. coli and insect expression platforms that deliver high quality recombinant antibody fragments, such as scFv, VHH, VH and Fab

FragPower Guaranteed Antibody Fragment Services

Fragment

Expression

Amount

Purity

Timeline

Deliverables

scFv

VHH/VH

Fab

E. coli

E. coli

Insect

10mg

10mg

3mg

≥90%

≥90%

≥90%

7 weeks

7 weeks

10 weeks

· Ab Fragment in buffered solution

· Optimized gene sequence report

· QC data

Applications:

The HTP gene to antibody service is designed to help researchers scale quickly from promising leads to purified recombinant antibodies in a microscale format to accelerate their preliminary drug screening process with below applications.

  • Epitope binning
  • Ligand competition binding studies
  • Affinity measurements
  • Primary & secondary screen assays
  • HTP biophysical characterization to assess developability, and more
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