Whats's New
EZ-Tn5™ Custom Transposome Construction Kit 
The INCOGNITO™ RNA Transcription Kits recently introduced by CELLSCRIPT™ is unique in what it brings for the process of in vitro transcription. The kit contains modified nucleosides, such as pseudouridine (Ψ) and/or 5-methylcytidine (m5C) in place of the corresponding U or C canonical nucleosides (Figure 1).
These mRNAs are also called "INCOGNITO mRNAs" since they do not initiate any innate immune responses like their analogous unchanged counterpart upon transfection into mammalian cells that express a wide range of RNA sensors.
It has been demonstrated that INCOGNITO mRNA, besides being less immunogenic, is translated into protein at much higher levels than the corresponding mRNA that does not contain modified nucleosides, both in cultured cells (Figure 2) and in whole organisms (Figure 3).
INCOGNITO mRNA have potential for therapeutic use in regenerative medicine such as tissue and organ repair, cell reprogramming, tissue engineering etc. by compensating the missing or defective protein once delivered to the cells in culture or in vivo.
References
- Karikó K, Buckstein M, Ni H, Weissman D. Immunity 23: 165–75, 2005. Suppression of RNA recognition by Toll-like receptors: The impact of nucleoside modification and the evolutionary origin of RNA. Journal Article
- Karikó K, Muramatsu H, Welsh FA, Ludwig J, Kato H, Akira S, Weissman D. Molecular Therapy 16: 1833–40, 2008. Incorporation of pseudouridine into mRNA yields superior nonimmunogenic vector with increased translational capacity and biological stability. Journal Article
- Anderson B. Muramatsu H, Nallagatla SR, Bevilacqua P. Sansing L, Weissman D, Karikó K. Nucleic Acids Res 38: 5884–92, 2010. Incorporation of pseudouridine into mRNA enhances translation by diminishing PKR activation. Journal Article
- E.g., United States Patent Application No. US 20090286852 Journal Article
- Warren L, Manos PD, Ahfeldt T, Loh YH, Li H, Lau F, Ebina W, Mandal PK, Smith ZD, Meissner A, Daley GQ, Brack AS, Collins JJ, Cowan C, Schlaeger TM, Rossi DJ. Cell Stem Cell. 7(5): 549–50, 2010. Journal Article
The following products are also offered:
INCOGNITO™ SP6 Ψ-RNA Transcription Kit
INCOGNITO™ T7 ARCA 5mC- & Ψ-RNA Transcription Kit
INCOGNITO™ T7 5mC- & Ψ-RNA Transcription Kit
For more check the following link http://www.cellscript.com/
INCOGNITO™ RNA Transcription Kits from CELLSCRIPT™
The EZ-Tn5 Custom Transposome Construction kit contains reagents to construct custom Transposons and Transposomes for both in vivo and in vitro mutagenesis.
Two different products are available in this category:
- EZ-Tn5 Custom Transposome Construction Kit (with pMOD-2 vector)
- EZ-Tn5 Custom Transposome Construction Kit (with pMOD-3 vector)
For details on the vector mapa and sequence please click here
Applications
- Creation of custom Transposomes for in vivo use (bacterial strain development, microbial transposon mutagenesis and gene expression studies, bacterial gene rescue
- Creation of custom transposons for in vitro mutagenesis of plasmid-borne genes and cloned open reading frames; Sanger sequencing of large insert plasmid clones, gene expression studies of cloned open reading frames in plasmid-based gene expression systems
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AACC Conference, Atlanta, GA and Rockville, MD July 24, 2011- OriGene Technologies, Inc., a gene-centric life sciencescompany known for its development cDNA clones,
human full length proteins, antibodies, and other molecular tools for multiplex research introduces its major breakthrough technology High-Density Protein Microarray Chip for the quality control of its (TrueMAB™) monoclonal antibodies/
The new chip enables OriGene to screen monoclonal antibodies against 10,000 human proteins to provide the most specific monoclonal antibodies.The antibodies validated with the OriGene High-Density Protein Microarray Chip will enable IVD manufacturers to develop the most accurate and specific immunoassays for clinical applications.
"We are extremely pleased with our progress in achieving this significant advancement in our Genome-Wide High Throughput Antibody Development Program. Better measurement equates to better science. We are confident this technology will advance research and the resulting analysis with validation superior to any other antibody manufacturer," commented Wei-Wu He, Ph.D., CEO & Chairman, OriGene Technologies.
To learn more about OriGene TrueMABs, visit OriGene at the upcoming AACC in Atlanta, GA from July 24 - 26 at Booth #4254 or go to www.origene.com.
About OriGene Technologies
OriGene Technologies, Inc., is a gene-centric life sciences company dedicated to support academic, pharmaceutical and biotech companies in their research of gene functions and drug discovery. OriGene develops proteins, antibodies, and other molecular tools to allow researchers to analyze their data on amultiplex level. OriGene's novel product lines include the world's largest cDNA and shRNA clonecollections, over 5,000 purified human proteins, ~ 7000 high quality antibodies, and 100,000 highly validated human tissues. OriGene alsoprovides a broad range of antibody validation products including genome-wide tagged antigen standards and extensive IHC slides derived from our tissue collection.
2,000 TrueMAB™ monoclonal antibodies made against native epitopes
With our March release of 208 new TrueMAB monoclonal antibodies, OriGene's TrueMAB offering now surpasses 2,000.
- Monoclonal antibodies made against protein antigens (primarily full-length proteins expressed in human cell lines) for native epitopes
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- 30% discount now at $209 per 100 uL (valid through end of March, 2011)
TrueMAB TM monoclonal antibodies are the next generation of antibodies. Contrary to conventionally generated antibodies with short peptide immunogens, each TrueMAB TM monoclonal antibody is specifically developed with full-length proteins produced from human cell lines. Therefore, TrueMAB TM antibodies provide superior sensitivity and specificity for immunoassays that require native epitope recognition.
53% of TrueMAB are qualified for IHC and flow applications, 65% for IF, and 97% for WB.
Sign up for our TrueMAB webinar at 2PM ET, Wed. Jul. 6, 2011
Hybridomas available for licensing
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Puromycin conjugates for in vitro and in vivo labeling of proteins
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| ProteinKinase | 235 |
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Webinar: Making smarter choices on cDNA clones
Clone a gene?
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Which is the best for me?
Sign up for our Webinar and get these questions answered.
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The most comprehensive cancer survey tissue microarray, period!
Made from high quality tumor tissues collected from US sites
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OriGene Acquires Blue Heron, the Gene Synthesis Expert
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Survey Gene Expression in Hundreds of Prostate Cancer Tissues
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Nextera™ Technology for Next-Generation Sequencing Library Preparation
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