BacuVance Baculovirus Expression Service
Deliverables:
- Construct report
- 5 ml, >108 pfu/ml virus stock
- Expression data
- Purified proteins
- *QC data- SDS-PAGE will be performed to determine protein purity. BCA or Bradford assay will be used to measure protein concentration and Western blot using anti-His or anti-GST antibodies will be performed, to determine protein identity. LC-MS/MS peptide mapping will be used in addition to Western Blot, to determine protein identity (if necessary)