BacPower™ Guaranteed Bacterial Protein Expression
Jan 20, 2017 |
Focus Of The Month
This service from our partner Genscript offers the complete solution from gene to protein.We offer Gene synthesis (codon optimized for expression in E.coli), subcloning into expression vector and all the way through protein expression and purification to provide you guaranteed protein amounts, purities, and low endotoxin levels. You have the option of producing His tagged or tag-free proteins to meet your research needs. You simply provide protein sequence and we will deliver the protein at the amount and purity that you specified all in a month’s time. If your target recombinant protein becomes insoluble in the inclusion body fraction, FoldArt™, our proprietary protein refolding technology will be applied to solubilize the protein at no extra cost to you. This service is also ideal for the production of high quality protein antigens for antibody production.
Service Details
| Steps |
Specification |
Timeline* |
Deliverables |
| Gene synthesis, Cloning, Plasmid Prep |
- Gene synthesis and Codon optimization
- Sub cloning into appropriate expression vector
- Plasmid Prep and DNA QC
|
2 weeks |
- Protein in buffered solution with your specified amount & purity – Guaranteed
- Optimized gene sequence report
- QC data
|
| Protein Expression Testing |
- Transform plasmids into appropriate bacterial expression strain
- Protein expression evaluation and optimization
|
1 week |
| Protein Purification, Tag Removal (Optional) & Refolding (Optional) |
- Scale up protein expression using optimized conditions
- Purification to reach desired protein amounts and purity
- Tag removal and separation of tag-free protein (if requested)
- Refolding in case of insoluble protein (performed only if required)
|
1-2 weeks |
| QC & Delivery |
- Purity analysis and Detection using SDS-PAGE and Western Blot (for tagged protein)
- Bradford assay for quantitation
- Protein delivery
|
1 week |
Terms and Conditions
- Protein sequences are subject to pre-project analysis
- Exclusions are applicable to membrane proteins, toxic proteins and for target proteins greater than 100kDa
- To increase the expression level of your protein, codon optimization using our proprietary, OptimumGene™ technology, will be performed during gene synthesis
- Insoluble proteins maybe subject to FoldArt™ technology, GenScript’s proprietary protein refolding platform
- In all cases, final protein will be guaranteed to meet customer specifications, failing which, any charge to the customer will be waived
