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LEXSY: Membrane Protein Expression & Crystallization

Crystal Structure of channelrhodopsin 2 from LEXSY

Integral membrane proteins are notoriously hard to express in the common recombinant expression systems. However, in a recent Science paper the eukaryotic system LEXSY was used to produce channelrhodopsin (ChR2): both its native form and a slow-mutant were expressed with yields in the mg per litre range, the recombinant proteins were crystallized.

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