Jun 7, 2017 | Newsletters
5-Hydroxymethylcytosine (5-hmC) research tools
The so-called “6th base” 5-hydroxymethylcytosine (5-hmC) is a DNA modification that is an important epigenetic marker associated with e.g. stem cell differentiation and brain development.
5-hydroxymethylated DNA fragments can be used as sequencing control[5-9] or for pull-down of 5-hmC-binding proteins from cellular lysate. They are typically generated by enzymatic incorporation of 5-hmdCTP.