Apr 25, 2017 | What's New
Origene has brought a novel technology for transgene integration via insertion into the AAVS1 locus.
Adeno-associated virus integration site 1 (AAVS1) in the human genome is a safe harbor for transgene integration via the CRISPR technology. It is transcriptionally active and transgene expression from this site is stable.
Features of AAVS1
AAVS1 Safe Harbor knockin system via CRISPR- This system needs two components:
This has been validated by cloning RFP into the AAVS1 donor vector and observing RFP fluorescence after transfection along with the Guide RNA in HEK293T cells.